Circular RNA pyridoxal kinase (circPDXK) involves in the progression of ovarian cancer and glycolysis via regulating miR-654-3p and hexokinase II

Abstract Background Circular RNA pyridoxal kinase (circPDXK; hsa_circ_0061893) is newly identified to be aberrantly expressed in ovarian cancer (OVCA); however, its functional role OVCA cells remains expounded. Methods Real-time quantitative polymerase chain reaction, western blotting, and immunohistochemistry quantified protein expression levels. MiRNA binding site prediction tools predicted direct interaction between two RNAs, dual-luciferase reporter immunoprecipitation assays further confirmed that prediction. Cell-counting kit-8, colony formation, 5-ethynyl-2ʹ-deoxyuridine measured cell growth; nude mice xenograft tumor experiment detected growth. Transwell Annexin V-fluorescein isothiocyanate/propidium iodide staining evaluated motility apoptosis. Glycolysis process was determined by glucose uptake, lactate, ATP assay kits. Results CircPDXK highly patients’ tissues cells, concomitant with microRNA (miR)-654-3p downregulation hexokinase II (HK2) upregulation. interference of circPDXK could restrain viability, DNA synthesis, migration, invasion, glycolysis but also retard Allied those are higher apoptosis rate, elevated Bax E-cadherin levels, depressed ki67 HK2 Compared inhibition, restoration miR-654-3p functions analogical effects vitro. Mechanistically, there interactions or HK2; moreover, inhibition weaken the roles either ectopic abrogates overexpression. Conclusion CircPDXK/miR-654-3p/HK2 axis a novel molecular mechanism progression glycolysis, targeting might overcome OVCA.